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YbaK protein domain : ウィキペディア英語版 | YbaK protein domain
In molecular biology, this protein domain of unknown function is found in numerous prokaryote organisms. This domain also occurs in a number of prolyl-tRNA synthetases (proRS) from prokaryotes. Thus, the domain is thought to be involved in oligonucleotide binding, with possible roles in recognition/discrimination or editing of prolyl-tRNA.〔 〕 ==Function== Studies have shown that YbaK functions as a Cys-tRNAPro deacylase ''in vivo'', deacetylation additionally involves turning genes off, hence, it can be assumed that it is preventing the addition of an amino acid to a tRNA molecule, thus preventing translation. ''In vitro'' studies with the full set of 20 ''E. coli'' aminoacyl-tRNAs revealed that the ''Haemophilus influenzae'' and ''E. coli'' YbaK proteins are moderately general aminoacyl-tRNA deacylases that preferentially hydrolyze Cys-tRNAPro and Cys-tRNACy. Furthermore, YbaK-mediated hydrolysis of aminoacyl-tRNA has been indicated to influence cell growth.〔 〕 It has been further indicated that YbaK domain is important in the editing function if the wrong amino acid has been joined to the wrong tRNA.
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